26 April 2017

The contest and participants...

The Trinity Access Programme (TAP) has strong links to a number of secondary schools in deprived areas of Dublin. A member of TAP staff (Dr Ronan Smith)  helped with arrangements. He contacted theTAP liason teacher in each of the schools listed in section 7.1.1.  They were invited to select one or two pupils in the transition year to attend a workshop in TCD on 23rd January 2017 where the NoMorFilm project was explained. There was a breakout session where the pupils interacted with postgraduate students and they were given guidance for 2 hours in composing a poster on the subject of penicillin. The workshop finished with a discussion about the posters and how they could have been imporved,  The students were given 4 weeks to research a topic of relevance to NoMorFilm and individually to produce a poster that was submiyyed as a pdf document one week in advance of the poster session.

The posters were printed and mounted on poster boards in the foyer of the Moyne Institute of Preventive Medicine, TCD. The best five were selected and the winners  invited to come to the TCD microbiology teaching  laboratory for  work experience.  All pupils who participated in the poster competition received a certificate. The results were posted on the “current news” section of the Department of Microbiology website.

Nineteen posters were submitted by pupils from ten schools by February 20th  2017.

The winners...

The poster presentation competition occurred in Moyne Institute TCD and the winners selected on March 2nd 2017.

The five best posters were produced by these winners:

Michael Makarenko       Terbinafine                               Balbriggan Community College

Stacey Torres               The antibiotic fidoxamicin          Mercy Secondary School

Ian Downey                  Lamivuine  Zidovidine               Ballinteer Community School

Emmet McDonald         Dapsone                                   Moyle Park College

             Steven Thomas            Tedizolid                                    Moyle Park College

The five winners at TCD contest.

The prize...

The students learned how to make agar plates, to culture bacteria, to stain cells by the Gram method and to observe bacteria by light microscopy. They performed experiments on hand washing, they isolated bacteria from the skin, nose and mouth using swabs, and from the environment on settle plates. They isolated coliforms from river water using the membrane filter technique. Staphylococcus aureus was selected on mannitol salt agar and tested for haemolytic activity and for antibiotic susceptibility / resistance. Oral streptococci  were isolated on  Mitis Salivarius agar. They were shown how to perform dilutions with Gilson micropipettes  using potassium permanganate coloured water and then they performed viable counts on a culture of bacteria by the serial dilution/plating method.

Susceptibility/resistance to antibiotics was determined by the disc method. Antibiotic resistant mutants of E. coli were isolated by selection on rifampicin agar. The frequency of mutation was calculated. Transfer of antibiotic resistance by plasmid-mediated conjugation was demonstrated.

Demonstrating microscopy.